TABLE 3.

Specificity of yellow fever virus 17D vaccine real-time RT-PCR assay on clinically relevant samples

Virusc	Sample type	PFU/ml	Result (Cq)a
			YFV17D	YFallb
17D vaccine	Serum		Positive (34.6)	Positive (35.0)
	Serum		Positive (35.8)	Positive (36.2)
	Serum		Positive (32.2)	Positive (33.6)
	Lung		Positive (25.8)	Positive (28.3)
	Liver		Positive (30.1)	Positive (31.3)
	Kidney		Positive (26.4)	Positive (27.7)
	Thymus		Positive (29.3)	Positive (29.9)
BA-55	Spiked serum	100,000	Negative	Positive (26.0)
		10,000	Negative	Positive (27.2)
		1,000	Negative	Positive (30.4)
FMD 1240	Spiked serum	10,000	Negative	Positive (22.9)
		1,000	Negative	Positive (26.4)
INHRR 10a-10	Spiked serum	100,000	Negative	Positive (20.9)
		10,000	Negative	Positive (23.3)
		1,000	Negative	Positive (25.3)

^aC_q, quantification cycle; average of two replicates.

^bPan-yellow fever primers described by Domingo et al. (26).

^cViruses are described in Table 1.