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Mass characterization of lipid A profiles in polymyxin-susceptible and -resistant P. aeruginosa strains. Wild-type PAK (A), PAKpmrB6 (B), and PAKpmrB12 (C) mutants and the predominant structures of lipid A from the wild-type PAK and/or both pmrB mutants (D). The lipid A samples were analyzed by LC-MS/MS in negative ion mode, which obtained most lipid A peaks with the loss of one hydrogen ([M-H]−). R-3-hydroxydecanoate is shown in blue and l-Ara4N is shown in red.
