FIG 5.

Perturbations of the intermediates in l-Ara4N synthesis pathway. UDP-glucuronate, UDP-l-Ara4N, and UDP-l-Ara4FN were detected using the HILIC method, whereas undecaprenyl phosphate-l-Ara4N was detected using the RPLC method. The green box indicates the metabolite that was significantly increased by >2-fold, while the red boxes show metabolites that were exclusively detected in the pmrB mutants compared to that in the wild-type PAK. Metabolites in gray boxes were not detected through either the HILIC or RPLC method. **, P < 0.01; ***, P < 0.001 (Student's t test).