FIG 2.

Triclosan enhances aminoglycoside killing of 24-h-old biofilms. Twenty-four-hour-old biofilms grown on minimum biofilm eradication concentration (MBEC) plates were treated for 6 h with 100 μM triclosan, 500 μM tobramycin, 100 μM gentamicin, or streptomycin, alone and in combination, and the number of viable cells within the biofilms was quantified using the BacTiter-Glo assay. The assay was performed at least three times in triplicate. The results represent the means ± the standard error of the mean (SEM). A one-way analysis of variance (ANOVA) followed by Bonferroni's multiple comparison post hoc test was used to determine statistical significance compared to each aminoglycoside alone (*, P < 0.05).