FIG 7.

Aminoglycosides combined with triclosan do not increase biofilm dispersal. (A) Twenty-four-hour-old biofilms grown on MBEC plates were treated with triclosan (100 μM), tobramycin (500 μM), gentamicin (100 μM), or streptomycin (100 μM), alone and in combination. The effect on biofilm biomass was quantified by staining with crystal violet. The experiment was performed at least five times in triplicate. The results represent the means ± SEM. A one-way ANOVA followed by Dunnett's multiple-comparison post hoc test was used to determine statistical significance compared to no treatment (*, P < 0.05). NS, not significant. (B) Twenty-four-hour-old biofilms grown in flow cells were treated with triclosan (100 μM), tobramycin (524 μM), or the combination for 6 h. Live cells are stained green, and dead cells are stained red. Representative images are shown: no treatment (top left quadrant), tobramycin alone (top right quadrant), triclosan alone (lower left quadrant), and a combination (lower right quadrant). Insets are shown for the live channel (A2, B2, C2, and D2) and for the dead channel (A3, B3, C3, and D3) for each condition.