TABLE 3.
MIC and inhibition of ATP synthesis (IC50) by TO, TO analogs, and reference compounds for different bacterial strains and mitochondriaa
| Compound | ΔhemB strain |
Newbould |
E. coli |
IC50 (μg/ml) for mitochondria | |||
|---|---|---|---|---|---|---|---|
| MIC (μg/ml) | IC50 (μg/ml) | MIC (μg/ml) | IC50 (μg/ml) | MIC (μg/ml) | IC50 (μg/ml) | ||
| TO | 0.06 | 18.5 ± 1.9 | >128 | 95.5 ± 13.7 | >128 | 264.73 ± 30.8 | >1,024 |
| FcM | 0.06 | 18.9 ± 3.6 | 2 | 40.2 ± 13.8 | 32 | 102.42 ± 16.6 | >1,024 |
| Fcm | 2 | 47.0 ± 9.6 | 8 | 111.0 ± 11.3 | 64 | 223.09 ± 45.9 | >1,024 |
| FC02-190 | 8 | 85.1 ± 7.0 | >128 | >1,024 | >128 | >1,024 | >1,024 |
| FC04-116 | >128 | >1,024 | >128 | >1,024 | >128 | >1,024 | >1,024 |
| DCCD | NDb | 1.44 ± 0.54 | ND | 1.32 ± 0.32 | ND | 1.62 ± 0.54 | 0.82 ± 0.17 |
| CCCP | ND | 1.46 ± 0.27 | ND | 6.11 ± 1.1 | ND | 2.80 ± 1.01 | 7.19 ± 2.23 |
| Oligomycin | ND | 8.67 ± 1.9 | ND | 9.41 ± 1.5 | ND | 5.63 ± 1.23 | 5.91 ± 0.82 |
| Tomatine | >128 | >1,024 | >128 | >1,024 | >128 | >1,024 | >1,024 |
| Bedaquiline | >128 | >1,024 | >128 | >1,024 | >128 | >1,024 | >128 |
| Gentamicin | 8 | >1,024 | 0.25 | >1,024 | 1 | >1,024 | >1,024 |
| Levofloxacin | 0.25 | >1,024 | 0.25 | >1,024 | 0.03 | >1,024 | >1,024 |
a
The “>” sign denotes the highest concentration tested.
b
ND, not determined.