FIG 2.

Effects of antiretroviral drugs on the expression of FGF21, KLB, CHOP10, and HSPA5 mRNAs in human SGBS adipocytes and on KLB promoter activity in adipogenic cells. (A) SGBS human preadipocytes were differentiated in culture into adipocytes in the presence of the following drugs: zidovudine (AZT), 50 μM; stavudine (D4T), 50 μM; efavirenz (EFV), 5 μM; rilpivirine (RPV), 10 μM; lopinavir-ritonavir 4:1 (LPV/r), 20 μM; raltegravir (RAL), 5 μM; elvitegravir (ELV), 5 μM. (B) SGBS human adipocytes were differentiated in culture into adipocytes and treated for 24 h with the following drugs: AZT, 100 μM; D4T, 100 μM; EFV, 20 μM; RPV, 15 μM; LPV/r, 20 μM; ELV, 20 μM; tunicamycin (TUN), 2 μM; thapsigargin (THAP), 2 μM. Data are presented as means ± SEM from 4 to 5 independent experiments and are expressed relative to values for control cells (defined as 1). (C) Luciferase activity in adipogenic HIB-1B cells transiently transfected with a plasmid construct in which luciferase is driven by the −1,055/+45 region of the KLB gene. Cells were treated with the following drugs: EFV, 50 μM; LPV/r, 20 μM; TUN, 2 μM; THAP, 2 μM; rosiglitazone (ROSI), 2 μM. Data are normalized to Renilla luciferase activity driven by the cotransfected pRL-CMV plasmid. *, P < 0.05; **, P < 0.01, and ***, P < 0.001 for each drug treatment versus control.