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Expression of SmeF by co-trimoxazole-resistant mutants. The level of expression of SmeDEF was measured by Western blotting using an anti-SmeF antibody. D457, wild-type S. maltophilia strain; D457R, SmeDEF-overexpressing mutant. In agreement with the real-time RT-PCR data, the mutants MBS510, MBS517, MBS518, and MBS522 overexpress SmeDEF.
