FIG 3.

Analysis of smeVWX expression by co-trimoxazole-resistant mutants. The level of expression of smeVWX was analyzed using a fluorescent reporter as described in Materials and Methods. MBS708, MBS709, MBS716, MBS718, and PBT06 were derived from MBS509, MBS512, MBS534, MBS560, and MBS287, respectively. In agreement with real-time RT-PCR data, the expression of the fluorescent reporter YFP was higher in these mutants than in the control strains PBT02 (a derivative of the wild-type strain D457) and MBS713 (derived from MBS520, a mutant that overexpresses SmeDEF but not SmeVWX).