TABLE 1.
Bacteria and plasmid used in this work
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| S. maltophilia | ||
| D457 | Clinical strain, wild type | 29 |
| MBS411 | D457 ΔsmeE | 11, 46 |
| PBT02 | D457 with pPBT04 plasmid | 31 |
| PBT06 | MBS287 with pPBT04 plasmid | 31 |
| D457R | D457 mutant overexpressing SmeDEF efflux pump | 29 |
| MBS287 | D457 mutant overexpressing SmeVWX efflux pump | 11 |
| MBS704 | D457 ΔsmeW | 31 |
| MBS509-MBS602 | Mutants isolated in different amounts of co-trimoxazole | This work |
| MBS708 | MBS509 with pPBT04 plasmid | This work |
| MBS709 | MBS512 with pPBT04 plasmid | This work |
| MBS713 | MBS520 with pPBT04 plasmid | This work |
| MBS716 | MBS534 with pPBT04 plasmid | This work |
| MBS718 | MBS560 with pPBT04 plasmid | This work |
| E. coli | ||
| CC118λpir | λpir lysogen from CC118, Tetr, Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 | 45 |
| 1047(pRK2013) | Strain containing the pRK2013 helper plasmid, Kanr | 47 |
| Plasmid | ||
| pPBT04 | Cloning vector pSEVA237Y containing smeVWX promoter region | 31 |