TABLE 2.
Expression levels of efflux pump genes smeV and smeD
| Strain | Phenotypea | Fold change ± SEMb |
|
|---|---|---|---|
| smeV | smeD | ||
| D457 | 1.00 ± 0.0 | 1.00 ± 0.0 | |
| D457R | 0.33 ± 0.03 | 6.99 ± 3.61 | |
| MBS509 | Q | 55.03 ± 9.40 | 0.80 ± 0.19 |
| MBS510 | Q/CHL/ERY | 0.87 ± 0.11 | 8.59 ± 1.55 |
| MBS517 | Q/ERY | 0.71 ± 0.17 | 7.54 ± 1.14 |
| MBS518 | Q/ERY | 0.64 ± 0.28 | 7.99 ± 1.58 |
| MBS522 | Q/CHL/ERY | 0.74 ± 0.26 | 5.46 ± 1.01 |
| MBS534 | Q | 30.41 ± 4.82 | 0.75 ± 0.10 |
| MBS560 | Q/CHL | 48.05 ± 19.48 | 0.58 ± 0.05 |
| MBS571 | Q/CHL | 91.20 ± 41.68 | 0.71 ± 0.09 |
a
Antibiotic resistance phenotype. Q, quinolone resistance; Q/CHL/ERY, quinolone, chloramphenicol, and erythromycin resistance; Q/CHL, quinolone and chloramphenicol resistance; Q/ERY, quinolone and erythromycin resistance.
b
Expression levels are fold change of each mutant versus the wild-type strain, D457. Data are the means from three independent experiments. Fold changes larger than five are highlighted in bold.