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RM-1-132-resistant clones. (A) Clones C-d12 and A-f8 show higher EC50s for RM-1-132. Following selection and cloning of transfectants, 2-day invasion/growth assays were performed as described in Materials and Methods. (B) Western blot demonstrating that mutant CDPK1s are not overexpressed. GFP is expressed from another locus and was used as a loading control. Cell numbers are in millions. Markers (M) are 70 (faint), 55, and 35 kDa, from top to bottom, respectively.
