FIG 4.

TSAs and CETSAs of CDPK1 mutant clones. (A) TSAs using purified recombinant CDPK1(L96P). Comparison of the top and bottom panels shows that the chelation of calcium or the addition of RM-1-132 stabilizes the mutant protein, although the stability remains lower than that of the WT (Fig. 1C). Fluorescence units (FU) are given in thousands. (B) CETSAs using each clonal line. (Left) L-CETSAs; (right) IC-CETSAs. CDPK1 clone A-f8 has the H201Q mutation, while clone C-d12 has the L96P mutation. Each symbol indicates the result of a separate duplicate assay.