TABLE 1.
Mutations found in the H*R strains
| H*R clone | Effect | Nucleotide change (5′→3′) | Amino acid change | Affected gene(s) | Locus tag(s) in referencesa | Product |
|---|---|---|---|---|---|---|
| RUCAMP18-1 | Stop gained | 383T→A | Leu128a | hpt | SAUSA300_0488/hpt; SACOL0554/hpt | Hypoxanthine-guanine phosphoribosyltransferase |
| RUCAMP18-2 | Missense variant | 500C→A | Ala167Asp | fruB/lacC_1 | SAUSA300_0684/fruB; SACOL0758/fruK | Fructose 1-phosphate kinase |
| RUCAMP18-3 | Missense variant | 317C→T | Thr106Ile | hpt | SAUSA300_0488/hpt; SACOL0554/hpt | Hypoxanthine-guanine phosphoribosyltransferase |
| RUCAMP18-4 | Stop gained | 847G→T | Glu283a | fruB/lacC_1 | SAUSA300_0684/fruB; SACOL0758/fruK | Fructose 1-phosphate kinase |
| RUCAMP18-5 | Frameshift variant | 106delG | Gly39fs | fruB/lacC_1 | SAUSA300_0684/fruB; SACOL0758/fruK | Fructose 1-phosphate kinase |
| RUCAMP19-1 | Stop gained | 730C→T | Gln244fs | purB | SAUSA300_1889/purB; SACOL1969/purB | Adenylosuccinate lyase |
| RUCAMP19-2 | Missense variant | 419C→A | Ala140Asp | hpt | SAUSA300_0488/hpt; SACOL0554/hpt | Hypoxanthine-guanine phosphoribosyltransferase |
| RUCAMP19-3 | Missense variant | 523C→T | Pro175Ser | fruB/lacC_1 | SAUSA300_0684/fruB; SACOL0758/fruK | Fructose 1-phosphate kinase |
| RUCAMP19-4 | Missense variant | 416G→A | Arg139His | purB | SAUSA300_1889/purB; SACOL1969/purB | Adenylosuccinate lyase |
| RUCAMP19-5 | Missense variant | 523C→T | Pro175Ser | fruB/lacC_1 | SAUSA300_0684/fruB; SACOL0758/fruK | Fructose 1-phosphate kinase |
| RUCAMP28-1 | Stop gained | 1860T→A | Tyr620a | relA | SAUSA300_1590; SACOL1689/relA2 | GTP pyrophosphokinase |
| RUCAMP28-2 | Missense variant | 461C→A | Ala154Glu | gmk | SAUSA300_1102/gmk; SACOL1221/gmk | Guanylate kinase |
| RUCAMP28-3 | Missense variant | 911C→T | Ala304Val | prsA | SAUSA300_0478/prs; SACOL0544/prsA | Ribose-phosphate pyrophosphokinase |
| RUCAMP28-4 | Missense variant | 394C→T | Arg132Cys | relA | SAUSA300_1590; SACOL1689/relA2 | GTP pyrophosphokinase |
| RUCAMP28-5 | Missense variant | 132T→A | Asp44Glu | prsA | SAUSA300_0478/prs; SACOL0544/prsA | Ribose-phosphate pyrophosphokinase |
| RUCAMP29-1 | SNP in IGR, putative regulatory region of gmk/SAUSA_1102 or SAUSA300_1101a | C→A (65 bp upstream gmk) | gmk | Guanylate kinase | ||
| RUCAMP29-1 | SNP in IGR, putative regulatory region of SAUSA300_1888 or the polycistronic transcript enrolling SAUSA300_1889/purBa | G→T (3 bp upstream ATG) | SAUSA300_1888 and/or purB | Conserved hypothetical protein/putative DNA-binding transcriptional regulator | ||
| RUCAMP29-2 | Missense variant | 265G→A | Ala89Thr | prsA | SAUSA300_0478/prs; SACOL0544/prsA | Ribose-phosphate pyrophosphokinase |
| RUCAMP29-3 | Missense variant | 307A→G | Thr103Ala | hpt | SAUSA300_0488/hpt; SACOL0554/hpt | Hypoxanthine-guanine phosphoribosyltransferase |
| RUCAMP29-4 | Stop gained | 2161A→T | Lys721a | relA | SAUSA300_1590; SACOL1689/relA2 | GTP pyrophosphokinase |
| RUCAMP29-5 | Missense variant | 650C→T | Ser217Leu | fruB/lacC_1 | SAUSA300_0684/fruB; SACOL0758/fruK | Fructose 1-phosphate kinase |