Figure 4.

Tobacco cell responses induced by cryptogein and by the mutated proteins. (A) Time course of ⁴⁵Ca²⁺ uptake. Cells were preincubated for 5 min in the presence of ⁴⁵Ca²⁺ before addition of elicitins (50 nM). (C) Correlation between the affinity of elicitins for sterols and the induction of Ca²⁺ influx with the use of the values obtained after a 60-min incubation period. (B) Effects of elicitin concentration on initial rate of extracellular medium alkalization. (D) Correlation between the affinity of elicitins for sterols and the extracellular medium alkalization induced with 20 nM elicitin. (⋄) control, ▴, cryptogein; ▵, X24; ▪, Y47F; □, Y47G; ○, Y87F.