TABLE 2.
Effects of increased extracellular [Na+] and osmolarity on potencies of KAE609, MMV011567, and CQ against P. falciparum parasitesa
| Parameter | Value |
||
|---|---|---|---|
| RPMI medium | High-Na+ RPMI medium | Sucrose RPMI medium | |
| Osmolarity (mosM) | 290 ± 3 | 349 | 349 |
| IC50 (nM) (n) | |||
| KAE609 against 3D7 parasites | 0.62 ± 0.04 (8) | 0.42 ± 0.03 (7) | 0.92 ± 0.13 (8) |
| P value | 0.0010 | 0.0497 | |
| KAE609 against Dd2 parasites | 0.9 ± 0.1 (5) | 0.6 ± 0.1 (5) | ND |
| P value | 0.03 | ||
| MMV011567 against Dd2 parasites | 285 ± 19 (5) | 178 ± 13 (5) | ND |
| P value | 0.002 | ||
| CQ against 3D7 parasites | 20 ± 1 (7) | 18 ± 2 (7) | 18 ± 1 (7) |
| P value | 0.1798 | 0.2106 | |
| CQ against Dd2 parasites | 93 ± 18 (5) | 106 ± 15 (5) | ND |
| P value | 0.6 | ||
a
The IC50 data are averaged from multiple (n) independent experiments, and errors denote SEM. The P values are from unpaired t tests and relate to the differences between the IC50s for parasites grown in RPMI 1640 medium and those grown in either high-Na+ RPMI 1640 medium or sucrose RPMI 1640 medium. CQ, chloroquine; ND, not determined.