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. 2018 May 25;62(6):e02629-17. doi: 10.1128/AAC.02629-17

FIG 2.

FIG 2

CrpP enzyme kinetics. (A) NADH oxidation rate comparisons for CIP (○) and NOR (●); 2.0 mM each antibiotic was incubated in the presence of 5.0 μg/ml His-CrpP protein at 37°C for 15 min. NADH oxidation was measured spectrophotometrically at 390 nm. (B) Michaelis-Menten plots for CIP (○) and NOR (●). The plot shows the NADH oxidation data. Data are means from four independent duplicate assays, with standard errors.