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RTD-1 rapidly induces intracellular ROS in C. albicans. DCFH-DA fluorescence was measured in drug-sensitive C. albicans ATCC 18804 and MDR ATCC 64124 after incubation with 6.25 μg/ml RTD-1 for the indicated intervals. The significance of the effect of RTD-1 treatment at all time points was a P value of <0.0001 (*; 2-tailed Student's t test). Experiments were performed using three biological replicates and repeated three times.
