Figure 2.

Determination of the transcriptional start site of the cpcA gene. Primer-extension experiments were performed with two cpcA-specific primers, BH80 and BH81, and 5 μg of total RNA. Both primers were also used for sequencing reactions to determine the transcriptional start site by comparison. The sequencing reaction performed with primer BH81 is presented. The primer extension experiment performed with BH80 is arranged to the sequence of BH81 without changing the position concerning the nucleotide sequence. The arrow indicates the transcriptional start site in the cpcA sequence.