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. Author manuscript; available in PMC: 2019 Jun 5.

Published in final edited form as: Eur J Pharmacol. 2018 Mar 3;828:9–17. doi: 10.1016/j.ejphar.2018.02.045

Fig 3 — Fig 3A. Spines on the dendritic branch of Golgi-stained neurons from WT (A), untreated (B), and 7,8-DHF- treated (C) experimental groups are shown (magnification 100x).

Fig 3B. Spine density was analyzed on order 2 and 3 dendritic branches of layer V cortical neurons in untreated WT, untreated 5xFAD and 7,8-DHF 5xFAD groups (n=10). Spine density was significantly lower in the untreated 5xFAD mice compared to WT mice. However, spine density in the DHF-treated mice was not significantly different than that in WT mice. *P< 0.05.

Fig 3C. Total spine number on branch order 2 and 3 in untreated WT, untreated 5xFAD and 7,8-DHF 5xFAD treated groups of Layer V cortical neurons. The total number of spines in the untreated 5xFAD group (n=10) significantly decreased compared to the WT group (n=10); *P< 0.05. DHF treatment prevented dendritic spine loss in 5xFAD mice, as the number of spines counted in the 5xFAD treated group (n=10) and WT (n=10) was not significantly different.

Fig 3D. The number of dendritic intersections at each radial distance from the soma for each neuron was counted and graphed as mean data per mouse treatment group within WT, untreated and 7,8-DHF treated groups. We performed a Sholl analysis to measure dendritic field density and structure changes between groups. Shown are the fits for the WT, 5xFAD and 5xFAD 7,8-DHF groups (n=10) using an asymmetric Gaussian lineshape. The curves fits were significantly different for 5xFAD vs. WT (P<0.05 using the R-factor ratio test). The 5xFAD 7,8-DHF curve approached significance compared to the 5xFAD curve (P<0.1) but there was no difference between the WT and 7,8-DHF-treated 5xFAD curves.

Fig 3 — Fig 3A. Spines on the dendritic branch of Golgi-stained neurons from WT (A), untreated (B), and 7,8-DHF- treated (C) experimental groups are shown (magnification 100x).

Fig 3B. Spine density was analyzed on order 2 and 3 dendritic branches of layer V cortical neurons in untreated WT, untreated 5xFAD and 7,8-DHF 5xFAD groups (n=10). Spine density was significantly lower in the untreated 5xFAD mice compared to WT mice. However, spine density in the DHF-treated mice was not significantly different than that in WT mice. *P< 0.05.

Fig 3C. Total spine number on branch order 2 and 3 in untreated WT, untreated 5xFAD and 7,8-DHF 5xFAD treated groups of Layer V cortical neurons. The total number of spines in the untreated 5xFAD group (n=10) significantly decreased compared to the WT group (n=10); *P< 0.05. DHF treatment prevented dendritic spine loss in 5xFAD mice, as the number of spines counted in the 5xFAD treated group (n=10) and WT (n=10) was not significantly different.

Fig 3D. The number of dendritic intersections at each radial distance from the soma for each neuron was counted and graphed as mean data per mouse treatment group within WT, untreated and 7,8-DHF treated groups. We performed a Sholl analysis to measure dendritic field density and structure changes between groups. Shown are the fits for the WT, 5xFAD and 5xFAD 7,8-DHF groups (n=10) using an asymmetric Gaussian lineshape. The curves fits were significantly different for 5xFAD vs. WT (P<0.05 using the R-factor ratio test). The 5xFAD 7,8-DHF curve approached significance compared to the 5xFAD curve (P<0.1) but there was no difference between the WT and 7,8-DHF-treated 5xFAD curves.