Sexually dimorphic cholinergic abnormalities in GW exposed mice. The effect of exposure to GW-related chemicals and stress on the levels of the ACh-synthesizing enzyme, ChAT, and GFP in the septum and hippocampus of CHGFP mice. Two-way ANOVA using the two brain regions, hippocampus and septum, as a repeated measure, revealed significant interaction terms between exposure and sex (p = 0.003 for ChAT; p = 0.016 for GFP assayed by western blot; and p = 0.025 for GFP determined by ELISA). This interaction is illustrated by the consistently diverging blue (male) and pink (female) lines for all of the measures.