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. Author manuscript; available in PMC: 2018 Nov 29.
Published in final edited form as: Mol Psychiatry. 2017 Nov 28;23(10):2057–2065. doi: 10.1038/mp.2017.230

Figure 2.

Figure 2

Early increased in oxidative stress and PV neurons and WFA/PNN deficit in the TRN of adult GCLM KO mice. (a) Micrographs show immunofluorescent labeling for 8-oxo-dG (green), WFA/PNN (blue) and PV neurons (red) in the TRN of P20 (Juvenile), P40 (Pubertal), and P90 (Adult) WT and GCLM KO mice. (b) The increased in 8-oxo-dG immunolabeling (in arbitrary unit, a.u.) in KO (red) was already present at P20, increased further in P40 and even higher at P90. (c) As the animal aged, the number of PV neurons decreased in TRN of KO compared to WT mice. (d) The number of WFA/PNN+PV neurons in the TRN of KO mice were also reduced in P40 and P90 when compared to WT mice. For each group, n = 4–5. Scale: 100 μm. Bars in all graphs represent SD. **p < 0.01; ***p < 0.001 (pair-wise Dunnett tests).