Fig. 2. High-fat-diet-induced increase in liver Tnfα transcript levels in tnf^+/+ mice.

Arithmetic means ± SEM (n = 7 mice per group, one replication) of relative hepatic (A; unpaired, two-tailed t-test) and adipose tissue (AT). (B; unpaired, two-tailed t-test with Welch’s correction) Tnfα mRNA abundance (relative to Gapdh or 18S mRNA) in tnf^+/+ mice on control diet and on high-fat diet (HFD) for 3 weeks. *p < 0.05