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. 2018 May 29;8:36. doi: 10.1038/s41387-018-0037-x

Fig. 4. High-fat-diet-induced FGF23 production was blunted in TNFα-deficient (tnf−/−) mice.

Fig. 4

Arithmetic means ± SEM of the serum intact FGF23 concentration (A; n = 3 tnf+/+ mice and 7 tnf−/− mice, one replication) and serum phosphate concentration (C; n = 11 tnf+/+ mice, and 8 tnf−/− mice, two replications) in tnf+/+ mice (white bars) and tnf−/− mice (black bars) before (control) and after 3 weeks of feeding a high-fat diet (HFD). Arithmetic means ± SEM of the serum calcitriol concentration (B; n = 8 mice per group) in a group of tnf+/+ mice (white bars) and tnf−/− mice (black bars) on control diet or HFD fed for 3 weeks. Arithmetic means ± SEM (n = 13 tnf+/+ mice and 12 tnf−/− mice, two replications) of the total body weight (D) in dependence of the duration of HFD and relative weight gain after 3 weeks of HFD (E); *p < 0.05, **p < 0.01, and ***p < 0.001 indicate significant difference between the genotypes; ###p < 0.001 indicates significant difference between control and HFD. (A–C: one-way ANOVA followed by Tukey’s multiple comparisons test; D: Holm-Sidak method; E: unpaired, two-tailed t-test with Welch’s correction)