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. 2018 May 22;11:166. doi: 10.3389/fnmol.2018.00166

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Copyright © 2018 Veszelka, Tóth, Walter, Tóth, Gróf, Mészáros, Bocsik, Hellinger, Vastag, Rákhely and Deli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Different types of brain endothelial and epithelial cell cultures examined by phase contrast microscopy and stained for tight junction proteins CLDN5 (red) and CLDN4 (green), and cell nucleus (blue). The CLDN5 immunostaining was well visible on the cell border of RBEC cells, while it was very weak or undetectable in the brain endothelial cell lines. MDCK and Caco-2 epithelial cells showed an intense staining for CLDN4. Bars: 50 and 100 μm (phase contrast pictures); 20 and 50 μm (immunostaining pictures).