Skip to main content
. 2018 May 22;11:165. doi: 10.3389/fnmol.2018.00165

Figure 1.

Figure 1

Simvastatin protected against 6-hydroxydopamine (6-OHDA)-induced cytotoxicity in SH-SY5Y cells. (A) SH-SY5Y cells treated with different concentration of 6-OHDA and cell viability determined after 24 h using a CCK-8 kit. (B) SH-SY5Y cells co-treated with 1 μmol/L simvastatin and 100 μmol/L 6-OHDA; cell viability determined after 24 h using a CCK-8 kit. (C) After 12 h co-treatment, expression of cleaved caspase-3 detected by western blot. (D) Relative quantitative analysis of cleaved caspase-3 expression. (E) Expression of Bcl-2 and Bax detected by western blot after 24 h co-treatment. (F) Analysis of the Bcl-2/Bax ratio. Data were obtained from three independent experiments. Statistical analysis was by Dunnett’s T3 test or least significant difference (LSD) post hoc test based on analysis of variance (ANOVA); data expressed as mean ± standard error of the mean (SEM). *P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.05.