Figure 9.

Simvastatin enhanced expression of anti-oxidative signaling molecules in midbrain samples in the unilaterally 6-OHDA-lesioned mouse model of PD. (A) Total protein in the midbrain was extracted with RIPA buffer, then western blot was used to detect the expression of GCLM and PGC-1α. Relative quantitative analysis of GCLM (B) and PGC-1α (C) expression performed using ImageJ. (D) Total SOD activity of midbrain samples determined using a Beyotime Total SOD Assay Kit; data were obtained from three independent experiments. Statistical analysis by Dunnett’s T3 or LSD post hoc test based on ANOVA; data expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ^#P < 0.05.