Figure 1.

Expression of a specific DLP1 mutant (DLP1-K38A) in cultured cells induces the formation of large DLP1-containing structures. Clone 9 cells were transfected to express either GFP-tagged DLP1-K38A (A) or untagged DLP1-K38A (C). Untagged DLP1 was detected by immunofluorescence microscopy with anti-DLP1 antibodies (C). Both untagged and GFP-tagged mutant DLP1 formed large fluorescent DLP1-containing structures (arrowheads). These were readily visible as dense structures in the cytoplasm by phase contrast microscopy (B and D, arrowheads). Bar, 10 μm.