Figure 6.

DLP1 assembles into macromolecular structures in a nucleotide-dependent manner. DLP1 assembles into sedimentable macromolecular structures (A). 6-His-tagged DLP1 was incubated with or without nucleotide and the reaction mixture was centrifuged at 100,000 × g to pellet assembled polymers. DLP1 polymer formation is apparently sensitive to GTP, because addition of GTP to the reaction mixture significantly reduces the amount of sedimentable DLP1 polymers (A). Furthermore, addition of GTPγS increases the amount of DLP1 in the polymeric form (A). Electron micrographs of GTPγS-treated DLP1 reveal clusters of rings as well as cylindrical structures, presumably stacks of rings (B and C), whereas no ordered structures are found either in the absence of nucleotide or in GTP-treated DLP1. Bars, 50 nm.