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. 2018 May 22;9:1073. doi: 10.3389/fimmu.2018.01073

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Copyright © 2018 Miller, Cosgrove, Wollman, Taylor, Zhou, O’Toole, Coles and Leake.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Single-molecule stoichiometry of CCL19-AF647. (A) Tracking of photoblinking Alexa Fluor 647 (AF647): localizations and intensity over time with sample images from the acquisition. (B) Distribution of apparent CCL19 foci stoichiometry (gray) overlaid with the predicted distribution based on randomly overlapping point spread functions (blue). (C) Kernel density estimates of intensity of AF647 labeled CCL19 in collagen (solid blue line), and under heparan sulfate immobilization (solid black line); CXCL13 in collagen (dotted blue line), and under heparan sulfate immobilization (dotted black line); and bovine serum albumin in 10% Ficoll (solid red line). All traces are normalized to the primary peak for clarity (see Supplementary Material).