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. 2018 May 22;9:1073. doi: 10.3389/fimmu.2018.01073

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Copyright © 2018 Miller, Cosgrove, Wollman, Taylor, Zhou, O’Toole, Coles and Leake.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparing fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS), and single-molecule tracking on BSA-AF647 in 10% Ficoll 400. (A) Single-molecule tracking: simplified schematic of the stages in tracking and the resulting fit with shaded regions indicating error bounds of one SD. (B) FRAP: schematic of technique, profile of bleached region in an immobilized sample, and example fluorescence intensity recovery trace. (C) FCS: schematic of the confocal volume, example section of intensity fluctuation trace, and correlation curve.