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. 2018 May 22;9:1073. doi: 10.3389/fimmu.2018.01073

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Copyright © 2018 Miller, Cosgrove, Wollman, Taylor, Zhou, O’Toole, Coles and Leake.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Single-particle tracking of chemokines in collagen. Second-harmonic imaging microscopy (SHIM) of collagen network in (A) 2D and (B) 3D. (C) Representative consecutive submillisecond images of chemokines in collagen. (D,E) Fitted diffusion coefficient distribution of CXCL13-AF647 and CCL19-AF647 showing mobile and immobile components in a collagen matrix with (F) just the fitted high-mobility diffusion coefficient distributions of CXCL13-AF647 (cyan) and CCL19-AF647 (magenta) (shaded areas indicate one SD).