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. 2018 May 22;9:1073. doi: 10.3389/fimmu.2018.01073

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Copyright © 2018 Miller, Cosgrove, Wollman, Taylor, Zhou, O’Toole, Coles and Leake.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal microscopy quantification of CXCL13-AF647 binding to lymph node follicles. (A) Schematic diagram of approximate locations of B-cell follicles in a wild-type murine lymph node. (B) Exemplar confocal microscopy images of CXCL13-AF647 and BSA-AF647 binding to lymph node tissue follicles (B220 + regions of lymph node tissue sections), and control with only B220 staining. (C) Quantification of the total fluorescent intensity for a fixed size imaging plane within a lymph node follicle. Each data point represents a distinct follicle.