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. 2018 May 22;9:1073. doi: 10.3389/fimmu.2018.01073

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Copyright © 2018 Miller, Cosgrove, Wollman, Taylor, Zhou, O’Toole, Coles and Leake.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Single-molecule analysis of CXCL13-AF647 in tissue. (A) Intensity average image of image acquisition to show autofluorescent extracellular matrix (ECM) in B220-stained B-cell follicle with no added chemokine. (B) Areas of (A) identified as ECM by segmentation with overlaid track localizations colored orange. (C) Intensity average image of image acquisition to show autofluorescent ECM in B220-stained B-cell follicle with added chemokine. (D) Areas of (C) identified as ECM by segmentation with overlaid track localizations colored by location on ECM (blue) or in the interstitial spaces between cells (cyan). (E) Comparison of diffusion coefficients of localizations in ECM locations in the presence (blue) and absence (orange) of CXCL13-AF647 (F) Comparison of diffusion coefficients for the ECM (blue) and chemokine (cyan) populations when tracking CXCL13-AF647 in lymph node tissue shown. (G) Distribution and fit of chemokine diffusion coefficients of CXC13-AF647 in tissue sections, shaded area indicates one SD.