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Measurements of the diffusion coefficient of Alexa-647 labeled BSA in 10% Ficoll 400.
| Condition | Diffusion coefficient (μm2s−1) | Number of measurements |
|---|---|---|
| Theoretical with stokes radius 3.48 nm | 12.3 ± 0.1 | |
| FCS | 18.8 ± 0.3 | 27 traces |
| FRAP | 7.1 ± 0.3 | 30 repeats |
| Single-molecule tracking | 9.3 ± 0.4 | 2,608 tracks (fitted 1,113 mobile tracks) |
Variation on the theoretical value is due to a potential ±2°C temperature change in the laboratory.
