Table 3.
AF647 labeled chemokine | Theoretical diffusion coefficients in water (μm2s−1) | Fitted diffusion coefficient (μm2s−1) | Error (μm2s−1) | Number of highly mobile tracks | R2of combined fit |
---|---|---|---|---|---|
CXCL13 | 149 | 6.2 | 0.3 | 1,930 | 0.980 |
CCL19 | 146 | 8.4 | 0.2 | 4,859 | 0.984 |
Optimized values were found by fitting a two gamma distribution to single-molecule tracking data.