Figure 4.

The ZFC3H1 Protein Is Required for RNA Foci Formation

(A) Dual SNHG19 and pA⁺ RNA-FISH analysis in the indicated factor-depleted HeLa cells. Merged images of DAPI (blue), SNHG19 (green), and pA⁺ RNA (red) are shown. Images from separate channels are shown in Figure S4B. pA⁺ RNA was detected with an oligo(dT) 50-mer probe.

(B and C) qRT-PCR analysis of SNHG19 RNA using total (B) or cytoplasmic and nuclear (C) fractions harvested from the indicated factor-depleted HeLa cells. In (C), 1 μg RNA from the respective fractions was used for reverse transcriptase reactions. Data are displayed as mean values, with error bars denoting SD (n = 4 biological replicates, except for siZCCHC8: n = 3). ^∗∗∗p < 0.01, Student’s t test; ns, not significant. Scale bars, 10 μm.

See also Figure S4B.