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. 2018 May 22;9:576. doi: 10.3389/fphys.2018.00576

FIGURE 3.

FIGURE 3

Dopamine neuron-selective unc-64 knockdown decreases DA release and DA uptake. Cultured dopaminergic neurons isolated from transgenic animals were used to measure the Amph-induced DA release (A) and DA uptake (B). (A) unc-64 silenced cells (pdat-1::unc-64 sas) show a statistically significant reduction of [3H]DA release (∗p ≤ 0.0001) with respect to control cells (pdat-1::kal-1 sas). t-Test was used to performed statistical analysis. Data are presented as the difference of dpm (disintegration per minute) between samples treated with Amph and samples treated with control solution. (B) pdat-1::unc-64 sas cells show a statistically significant 30% reduction of [3H]DA uptake (∗p ≤ 0.001) with respect to control neurons (pdat-1::kal-1 sas). A similar reduction (40%) was observed in cells silenced for dat-1 (pdat-1::dat-1 sas). Statistical analysis was performed using one-way ANOVA and Bonferroni post-test. Both data are average of values obtained from three independent experiments.