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. 2018 May 22;9:1115. doi: 10.3389/fimmu.2018.01115

Figure 5.

Figure 5

Intracellular localization of TA-Ag/AuNPs. The Manders’ coefficients for co-localization of TA-Ag/AuNPs and cytoplasm (A) or lysosomes (B) in JAWS II cell culture exposed to 10 nm, 37 nm, 59 nm TA-AgNPs and 10 nm, 34 nm, 62 nm TA-AuNPs for 24 h at 2.5 µg/ml. *Significant differences with p ≤ 0.05. (C) Representative images for lysosomes (green), nanoparticles (NPs) (red) and nuclei (blue) in cells exposed to NPs, as described above. (D) NPs content in cells subjected to pretreatment with 10 µg/ml monodansyl cadeverine and 5 µg/ml cytochalasin D, and then to incubation with TA-Ag/AuNPs at 2.5 µg/ml for 6 h.