Figure 3.

Increased density of splenic macrophage subsets is not due to increased recruitment from bone marrow. (A) Spleen sections from naïve CCR2^RFP/+ mice or CCR2^RFP/+ recovered 2 days after MCAO to determine number and location CCR2⁺ (red; RFP) cells infiltrating from bone marrow (naïve n = 4, MCAO + 2 days n = 4). Flow cytometric analysis of spleens from CCR2^RFP/+ mice shows no difference in the percentage of (B) MOMA-1⁺; (C) MARCO⁺; (D); CD11b⁺; or (E) F4/80⁺ cells expressing RFP in mice recovered 2 days after MCAO in comparison to sham-operated animals (sham n = 4, MCAO n = 6). (F) Increased immunofluorescent labeling of proliferation markers (white, KI-67) associated with MARCO⁺ marginal zone macrophages (green, MARCO) or red pulp macrophages (red, F4/80) in the spleens of mice recovered 2 days after MCAO in comparison to sham-operated animals (sham n = 4, MCAO n = 4). Scale bars (A) 100 µm, (F) 50 µm. Data show mean ± SD; unpaired t-test.