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. 2018 May 22;9:1108. doi: 10.3389/fimmu.2018.01108

Figure 5.

Figure 5

Marginal zone macrophages (MZM) efficiently traffic antigen from the circulation to the follicle. (A) Fluorescently labeled model antigen (green, Dextran-FITC) is trapped in the spleen by MZM (red, MARCO) in spleens from animals recovered 2 days from middle cerebral artery occlusion (MCAO) or sham surgery or naïve controls 1 h after iv injection. By 24-h post iv injection, antigen has been trafficked into the follicle of the white pulp (WP). Naïve animals were injected with vehicle (PBS) as a control. Quantification of the percentage area of Dextran-FITC associated with the marginal zone (MZ) or WP of the spleen at 1 h (B) and 24 h (C) shows increased trapping of antigen in the MZ at 1 h and no impairments in antigen trafficking to the follicle at 24 h in mice 2 days after MCAO in comparison to sham or naïve controls (for each time point naïve n = 4, sham n = 2, MCAO n = 4, naïve + PBS n = 2). Scale bar 50 µm. Data show mean ± SD (B,C), **P < 0.01; one-way analysis of variance with Bonferonni correction.