Figure 4.

Protein levels of key components of the endogenous protective RISK and SAFE pathways. Quantification of the levels of effector proteins from the RISK pathway [Akt, phospho-Akt/Total Akt Thr308 (A), phospho-Akt/Total Akt Ser473 (B)] and the SAFE pathway [STAT3, phospho-STAT3/Total STAT3 tyr705 (C)] in gastrocnemius muscle from sham-operated, vehicle-treated (nCON) or streptozotocin-treated rats (dCON), and after ischemia reperfusion in vehicle-treated (nIR) and streptozotocin-treated rats (dIR). GAPDH was used as an internal control for all proteins. Data are expressed as the mean ± SEM of the intensity of the bands reported to the intensity of the internal control. *p < 0.05; **p < 0.01, ^ap < 0.05 for diabetes effect; ^#p < 0.05 for interaction.