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. 2018 May 22;9:579. doi: 10.3389/fphys.2018.00579

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Copyright © 2018 Pottecher, Adamopoulos, Lejay, Bouitbir, Charles, Meyer, Singer, Wolff, Diemunsch, Laverny, Metzger and Geny.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protein levels of GSK-3β and cleaved caspase-3. Quantification of the levels of (A) GSK-3β, (GSK-3β, phospho-GSK-3β/Total GSK-3β Ser9) and (B) caspase-3 effector of apoptosis in gastrocnemius muscle from sham-operated, vehicle-treated (nCON) or streptozotocin-treated rats (dCON), and after ischemia reperfusion in vehicle-treated (nIR) and streptozotocin-treated rats (dIR). GAPDH was used as an internal control except cleaved caspase-3 for which tubulin was used. Data are expressed as the mean ± SEM of the intensity of the bands reported to the intensity of the internal control. *p < 0.05, **p < 0.001, ****p < 0.0001, ^ap < 0.05 for diabetes effect; ^bp < 0.05 for IR effect; ^#p < 0.05 for interaction.