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. 2018 May 22;9:528. doi: 10.3389/fphar.2018.00528

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Copyright © 2018 Moreira, Silva, Silva, Lemos and Cortes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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D-pinitol activates the endothelial nitric oxide synthase (eNOS) phosphorylating and dephosphorylating its respective activation and inactivation sites. Western-blots for time-course of the phosphorylation level of Ser1177 (A), the activation site of eNOS, and the dephosphorylation level of Thr495 (B), the inactivation site of eNOS, were performed in endothelium-intact mesenteric arteries, in the absence (basal) and presence of D-pinitol (20 μM). All results are expressed as mean ± SEM of five experiments. ^∗P < 0.05 and ^∗∗P < 0.01 versus basal.