Table 2. Comparison of time of collection of sample with the diagnostic validity of LFI and molecular detection.

Sample collection	N (N*)	IHA-positive [n (%)]	LFI-positive [n (%)]	qRT-PCR-positive [n (%)]
				T3SS1	IpxO
1–7 days before the positive culture	4 (3)	1 (25)	3 (75); 3 (100)	1 (25)	1 (25)
On the same day as the positive culture	27 (24)	3 (11)	9 (33.3); 9 (37.5)	3 (11.1)	7 (26)
1–4 days after the positive culture	14 (13)	3 (21.4)	4 (28.6); 4 (30.8)	5 (35.7)	4 (28.5)
Total	45 (40)	7 (15.5)	16 (35.5); 16 (40)	9 (20)	12 (26.7)

* N and italicized numbers based on 40 functional LFI tests.