Fig. 5.

Replication is severely decreased in WR recombinants with large deletions at the left and right end. Growth of viruses on the indicated cell lines was measured at 48 h p.i. after low m.o.i. (0.01) infection. The titre of (a) cell-associated virus and (b) virus released into the supernatant was determined by plaque assay on BS-C-1 cells with immunostaining. Data are mean ± sem from three independent wells. (c) The sum of results in (a, b) presented as the total fold increase of virus at 48 h p.i. over the inoculum.