Fig. 7.

Restoration of C11R rescues the replication and plaque formation defect of WRΔLΔITRr. Virus production and plaque formation by WRΔLI/C11RC10L, WRΔLI/C11R and WRΔLI/C10L was compared with the parental strain, WRΔLΔITRr and WRΔLeft. The amount of (a) cell associated virus and (b) virus released into the supernatant after 48 h following a low m.o.i. (0.01) infection. Data are mean ± sem of three independent wells. (c) Area of immunostained plaques formed on BS-C-1 cells over 72 h. Horizontal lines indicate mean plaque area. Data are representative of two independent experiments. WRΔLeft and the other C11R-containing viruses were significantly different from the two viruses lacking C11R (P < 0.0001). (d) Summary of the genotypes of the recombinant viruses.