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. 2018 Mar 26;209(2):409–423. doi: 10.1534/genetics.118.300823

Figure 6.

Figure 6

FNCM-1 and FCD-2 are necessary for maintaining H3K4 dimethylation levels. (A) Top: Western blot analysis comparing H3K4me2 levels with histone H3 and α-tubulin antibodies for the indicated genotypes either in the absence or presence of HU (3.5 mM). Bottom: Quantitation of H3K4me2 levels normalized against either histone H3 or α-tubulin. Signal intensity was measured with GelQuant.NET. Numbers represent average for data from three independent experiments. SEM values are presented in parentheses. (B) Western blot analysis comparing the levels of SPR-5 normalized against α-tubulin for the indicated genotypes upon absence or presence of 3.5 mM HU treatment. α-tub, α-tubulin; wt, wild type.