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. 2018 Apr 3;209(2):523–535. doi: 10.1534/genetics.118.300977

Figure 2.

Figure 2

Mutations in the ERK MAPK pathway suppress activated Gq. (A) Mutations in the MAPKKK lin-45/Raf and the MAPK mpk-1/ERK suppress the coiled posture of egl-30(tg26) worms. Partial loss of Ras let-60 activity does not suppress activated Gq posture. (B) Waveform quantification of ERK pathway mutants. Mutations in lin-45, mek-2, and mpk-1 suppress activated Gq egl-30(tg26) similar to ksr-1 mutations. N ≥ 12, *** P < 0.001, one-way ANOVA with Bonferroni’s post hoc test. (C) Signaling components upstream of Raf do not suppress activated Gq. Mutations in the EGF ligand (lin-3) or the EGFR (let-23) do not affect the exaggerated waveform of egl-30(tg26) animals. The Ras partial loss-of-function mutation let-60(n2021) does not suppress activated Gq waveform. N ≥ 12, *** P < 0.001, one-way ANOVA with Bonferroni’s post hoc test. (D) The let-60(sy93) dominant negative mutation in Ras does not suppress activated Gq waveform. N ≥ 13, n.s., not significant, one-way ANOVA with Bonferroni’s post hoc test.