HIS-72 and HIS-74 are expressed in all germ cells, whereas HIS-70 is male germ line-specific. (A–C) Hermaphrodite adult stage. HIS-72 (A) and HIS-74 (B) expression shown in pachytene, oocytes, and sperm. In pachytene nuclei, the GFP signal is chromatin associated, but appears depleted from parts of the chromatin (arrows). In oocytes, strong nucleoplasmic signals obscure the chromatin-associated signal. (C) HIS-70 is only detectable in sperm. (D–F) Hermaphrodite L4 larval stage. HIS-72 (D) and HIS-74 (E) expression is present in all germ cells, including pachytene and spermatocyte stages. (F) HIS-70 is only detectable in spermatocytes, but absent from pachytene. (G–I) Male adult stage. HIS-72 (G) and HIS-74 (H) expression is visible in all germ cells, including pachytene stage and in spermatocytes and sperm. (I) HIS-70 is only detectable in spermatocytes and sperm. Bars represent 5 µm. Cartoon images of germ lines highlight the regions shown in (A–I).